Fig. 4

Multiplex PCR for An. culicifacies and An. fluviatilis sibling specific genes. a An. culicifacies: Lane 1,2 & 4: A/D species common product (382 bp), Lane 3: A/D species-specific product (313 bp), Lane 5: negative control, and Lane 6: 100 bp ladder. b An. culicifacies: Lane 1: negative control, Lane 2: 100 bp ladder, Lane 3: B/C/E species common product (133 bp & 385 bp) and Lane 4 & 5: B/C/E specific product (385 bp). c An. fluviatilis: Lane 1, 5 & 6: Sibling species S (295 bp), Lane 2: ladder (100 bp), Lane 4: Sibling species T (128 bp) and Lane3: Sibling species U (375 bp)