Discovery of antimalarial drugs from secondary metabolites in actinomycetes culture library

Table 1 In vitro antimalarial activity and cytotoxicity of actinomycete secondary metabolite extracts

	SN	Strain name	Bacteria name	IC₅₀		CC₅₀	SI		RI
	SN	Strain name	Bacteria name	3D7	Dd2	CC₅₀	3D7	Dd2	RI
Positive control (µM)	CQ	NA	NA	0.0134 ± 0.0004	0.191± 0.08	>5	>373	>26	1.29
Positive control (µM)	AS	NA	NA	0.0027± 0.0014	0.003 ± 0.0004	2.17 ± 1.26	803	723	14.24
Extracts (µg/mL)	2	HUT6003	Streptomyces aureus	0.39 ± 0.14	0.26 ± 0.13	0.35 ± 0.02	0.9	1.34	0.87
	7	HUT6034	Streptomyces verne	3.37 ± 0.3	3.90 ± 0.44	>50	>14.83	>12.84	1.16
	8	HUT6035	Streptomyces antibioticus	0.09 ± 0.03	0.22 ± 0.13	16.3 ± 5.39	188	73.7	2.44
	10	HUT6046	Streptomyces albus	6.93 ± 6.77	13.87 ± 0.60	0.27 ± 0.15	0.04	0.019	2
	16	HUT6124	Streptomyces bostroemi	2.9 ± 0.4	1.43 ± 0.30	13.74 ± 3.5	4.6	9.59	0.49
	19	HUT6190	Streptomyces lusitanus	2.8 ± 0.9	1.62 ± 0.21	4.51 ± 0.8	1.6	2.79	0.58
	23	JCM4193	Streptomyces ramulosus	5 ± 2.1	2.72 ± 0.08	9.97 ± 0.3	1.99	3.67	0.54
	26	GK3	Streptomyces sp.	0.97 ± 0.3	0.72± 0.12	1.1 ± 0.1	1.13	1.51	0.74
	27	GK7	Streptomyces sp.	0.36 ± 0.2	0.21 ± 0.11	0.31 ± 0.2	0.88	1.45	0.58

The in vitro antimalarial activity of actinomycetes secondary metabolites extracts against chloroquine/mefloquine-sensitive (3D7) and-resistant (Dd2) strains of P. falciparum, and cytotoxicity against Adult Mouse Brain Cells (AMB). The value of IC₅₀, CC₅₀, and SI is taken from two independent experiments performed in duplicate. SN sample number, CQ Chloroquine, AS Artesunate, IC₅₀ 50% inhibitory concentration, CC₅₀ 50% cytotoxic concentration, SI selectivity index (CC₅₀/IC₅₀), 3D7 Chloroquine/Mefloquine-sensitive strain of P. falciparum, Dd2 Chloroquine/Mefloquine-resistant strain of P. falciparum, RI resistance index (3D7 IC₅₀/Dd2 IC₅₀)

ISSN: 1349-4147