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Fig. 2 | Tropical Medicine and Health

Fig. 2

From: Development of two multiplex PCR assays for rapid detection of eleven Gram-negative bacteria in children with septicemia

Fig. 2

Gels showing specificity of developed Mpcr. a Multipex A: l = Ladder, Lane 1. DNA mix, Lane 2 (Vibrio cholerae, 883 bP), 3. Acinetobacter baumanii (320 bp), 4. Salmonella spp. (284 bp), 5. Pseudomonas aeruginosa (190 bp), 6. E. coli (128 bp), 7–11. Off targets (Table 1) 12. Negative control. b Multiplex B: L = Ladder, Lane 1 Mixed DNA, 2. Enterobacter spp. (1181 bp), 3. Aeromonas spp. (891 bp), 4. Camphylobacter spp. (855 bp), 5. Providentia alcalifacience (515 bp), 6. K. pneumoniae (174 bp), 7–11. Off targets (Table 1) 12. Negative control

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