Fig. 1

Gels showing target genes amplified at the optimized annealing temperature (55 °C). a Multipex A: M = Marker, Lane 1 (Vibrio cholerae, (883 bP), 2. Acinetobacter baumanii (320 bp), 3. Salmonella spp. (284 bp), 4. Pseudomonas aeruginosa (190 bp), 5. Shigella spp. (128 bp), 6. E.coli(128 bp), 7. Negative control. b Multiplex B: M = Marker, 1. Enterobacter spp. (1181 bp), 2. Aeromonas spp. (891 bp), 3. Camphylobacter spp. (855 bp), 4. Providentia alcalifacience (515 bp), 5. K. pneumoniae (174 bp), 6. Negative control